RT PCR test with melting analysis for detection of 16 osteoporosis- related genes mutations: COL1A1: -1997 C>A, COL1A1: 1546 (6252) G>T [Sp1 S>s], CYP19A1: A>G [rs2414096], CYP19A1: C>T [rs936306], ESR1: -397 T>C [PvuII], ESR1: -351 G>A [XbaI], IL6: -174 G>C, LRP5: 1999 G>A (Val667Met), LRP5: 3989 C>T (Ala1330Val), RANKL: C>T [rs9594738], RANKL: C>T [rs9594759], TNFRSF11B (OPG) : 245 A>C, TNFRSF11B (OPG) : A>G [rs4355801], TNFRSF11B (OPG) : 163 (160) T>C, VDR: 283 A>G (Bsml), VDR: 2 A>G (Lys2Arg) [FokI]

kit-pcr-detection